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| Vorphal,María Alejandra; Bruna,Carola; Wandersleben,Traudy; Dagnino-Leone,Jorge; Lobos-González,Francisco; Uribe,Elena; Martínez-Oyanedel,José; Bunster,Marta. |
| Abstract Backgroud Ferredoxin NADP(H) oxidoreductases (EC 1.18.1.2) (FNR) are flavoenzymes present in photosynthetic organisms; they are relevant for the production of reduced donors to redox reactions, i.e. in photosynthesis, the reduction of NADP+ to NADPH using the electrons provided by Ferredoxin (Fd), a small FeS soluble protein acceptor of electrons from PSI in chloroplasts. In rhodophyta no information about this system has been reported, this work is a contribution to the molecular and functional characterization of FNR from Gracilaria chilensis, also providing a structural analysis of the complex FNR/Fd. Methods The biochemical and kinetic characterization of FNR was performed from the enzyme purified from phycobilisomes enriched fractions. The... |
| Tipo: Journal article |
Palavras-chave: Ferredoxin NADP+ reductase; Ferredoxin; Sequence; Kinetic parameters; Structural features. |
| Ano: 2017 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100228 |
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| Bravo,Soraya; Parra,María José; Castillo,Rosario; Sepúlveda,Francisco; Turner,Aileen; Bertín,Ariana; Osorio,Germán; Tereszczuk,Joanna; Bruna,Carola; Hasbún,Rodrigo. |
| ABSTRACT The present work explores in vivo physiological, morphological and chemical features during full hydration, desiccation and rehydration of the filmy fern Hymenophyllum dentatum with two main objectives: 1) to get further insight about the mechanisms underlying its desiccation tolerance, and 2) to understand how this plant manages mechanical stress induced by water loss and recovery. With these purposes, physiological (relative water content and F /F chlorophyll fluorescence parameter), morphological (Confocal Laser Scanning Microscopy and 3D reconstruction) and chemical (FT- IR microspectroscopy) data were obtained and compared between fully hydrated, desiccated and rehydrated tissues of H. dentatum. Remarkable changes in cell architecture and... |
| Tipo: Journal article |
Palavras-chave: Hymenophyllum; Desiccation tolerance; Confocal 3D microscopy; FT-IR microspectroscopy; Rapid dehydration/rehydration. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-66432016000200402 |
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